DTU Multi Assay Core
DTU Multi Assay Core (DMAC) offers next generation sequencing services for industry and universities.
DMAC is a technology core that supports teaching and research activities at DTU. The core offers a wide range of collaborative and scientific services.
We provide complete NGS solutions from our state-of-the-art laboratory and offer both standard and customized services for NGS sample preparation for:
- A variety of DNA and RNA applications
- Single Cell Analysis on the 10X Genomics Chromium platform
- DNA and RNA Quality Assessment
- Microarray-based transcriptome profiling upon request
In addition to our wet lab services, we give advice on experimental design and basic bioinformatics assistance and training.
DMAC provides the following applications in a single- or a pair-end sequencing mode, including multiplexing:
Transcriptome sequencing is also called RNA sequencing (RNA-Seq). It is a method for investigating the transcription qualitatively as well as quantitatively. This method can provide knowledge of gene structure and levels in a given situation.
- Single cell RNA sequencing: An analysis where we analyse gene expression levels on a single cell resolution from a heterogenic cell population. We are using the 10x Genomics Chromium platform that provides transcriptional profiling of thousands of cells simultaneously
- Standard mRNA-Seq: The most widely used RNA-sequencing method for gene expression profile, enabling the analysis of coding (mRNA)
- Total RNA-Seq: Allows analysis of both coding (mRNA) and non-coding (incRNA) RNA, splices variant and translocations.
Whole-genome sequencing (WGS) is a comprehensive method for analysing entire genomes. Genomic information has been instrumental in identifying inherited disorders, characterizing the mutations that drive cancer progression, and tracking disease outbreaks.
We are manly focusing on the smaller genomes such as bacteria, fungus and viral genomes.
Amplicon sequencing allows the detection of low frequency variants quantitative analysis in a mixed population. It enables analysis of a high number of samples, simultaneously.
We offer an amplicon sequencing of your region of interest or our standard 16s rRNA v3-v4 region.
Whole Exome Sequencing (WES) provides you with the opportunity to investigate genetic variation underlying cancers, Mendelian and other complex human disorders. Exome sequencing only targets protein coding regions and provides a more cost-effective method compared to Whole Genome Sequencing.
Custom sequencing capture allows you to sequence your region of interest with high accuracy, in a high throughput and cost-effective manner.
The infrastructure at DMAC includes:
- Bioanalyser
- Nanodrop
- Qubit
- Tecan plate reader
- Bravo robotics
- 10x Genomic Chromium
- Ion TorrentS5