Research Group

Nanofluidics and bioimaging

Group leader: Rodolphe Marie

The Nanofluidics and Bioimaging group is part of the OASIS section.

As an interdisciplinary research group, we focus on experimental nanofluidics for the manipulation and imaging of biologically relevant particles such as macro-molecules, lipid vesicles, metaphase chromosomes and cells.

Our goal is to provide new tools for the life sciences based on a combination of micro-/nanofluidics, biophysics, and several optical microscopy techniques.

Applications

  • Enzymatic synergy
  • Liquid biopsy
  • Colloids
  • Cancer study
  • Cellular transport
The group as in 2022 with guests .... 

Projects

Ongoing research projects.

Single molecule microscopy of enzymatic synergy

NNF BioSAP grant

Cellulose fibers (grey) and cellulases fluorescence (red).

Single molecule localization microscopy of enzymes

We study the behaviour of cellulases enzymes on cellulose nanofibers using TIRF microscopy.

 

Collaboration with:
Jonas Nyvold Pedersen at DTU Health Tech.
Peter Westh at DTU Bioengineering.
Kim Borch at Novozymes.


Next generation biocolloids analysis for diagnostics

NNF Nerd grant

Nanofluidic platform based on an injection moulded single use device.

Nanofluidic platform for the isolation and study of biocolloids

Nanofluidics and microfluidics for the purification of biocolloids from liquid biopsy. Fluorescence microscopy and label-free microscopy.

 

Single Molecule microscopy of colloids

Part of the EU ITN Supercol.

Single molecule localization microscopy is used to image biomolecules on dielectric and metallic nanoparticles. 
As part of the project EU ITN Supercol, we use single molecule localization microscopy, one of the super-resolution microscopy approaches, to study the functionalization of colloids with DNA and proteins. In particular we study how dielectric colloids may disturb the imaging using experimental and numerical approaches.

Microfluidic platform for cancer cells in gradients

Part of the NNF synergy Migragen.

Detail of a microfluidic mixer device.

A reversible microfluidic platform for gradients

We design and fabricate a microfluidic platform able to create multiple chemical gradients over cells. The platform has a reversible sealing to allow for further analysis of the cells. It started as the project Micrograd.

In collaboration with:
Stine Falsig Pedersen (Copenhagen University).
Albin Sandelin (Copenhagen University).
Utte Resch-Genger (BAM, Berlin).

Microscopy for peptide transport studies

part of the NNF challenge center Citbio.

DMD-based structured illumination microscopy was developed for tracking single molecules.

As part of the NNF challenge center Citbio, we use live-cell microscopy of lipidated peptide transport in gut epithelial models.

Optical mapping of DNA in nanofluidics

Using nanofluidics to visualize fluorescence barcodes on genomic DNA. Combined with microfluidics to trap single cells and extract the genomic DNA.

Composite image of metaphase chromosomes and genomic DNA with denaturation barcodes, random and repetitive.

See our past work in the EU project Cellomatic under 'Publications'.

Rodolphe Marie received his PhD from DTU in 2004 where he worked with microcantilever sensors in the group of Anja Boisen at the Department of micro and nanotechnology. He was a Post Doctoral fellow in Lund, Sweden, at the department for solid-state physics with Jonas Tegenfeldt and Fredrik Höök working with microfluidics and localized plasmon sensors. Back at DTU in 2008 he joined the group of Anders Kristensen where he worked with nanofluidics. He was appointed Assistant Professor at DTU Nanotech in 2008, Associate Professor in 2012 and group leader in 2017.

PhD

PhD

PhD

Pavlina Senikoglou

Pavlina Senikoglou PhD student Department of Health Technology

Former PhD students

Adam C. Hundahl
Martin K. Rasmussen

Former MSc students

Philipp Willmer
Sofie Tidemand-Lichtenberg
Lu Chen