Research Group

Biophotonic Imaging

Group leader: Peter E. Andersen

Our aim is to drive cutting-edge research in multimodal, functional optical bio-imaging, and to establish a biophotonic imaging resource for translational research within DTU Health, where new optical technology enables new clinical achievements and improves healthcare.

Optical imaging offers great promise for addressing unmet clinical needs due to the combination of non-invasive, real-time capture of biomedical information enabling point-of-care decisions. This enables earlier onset of treatment, reduced therapy costs, reduced recurrence rates, improved clinical outcomes, and a better patient experience. Moreover, in biology applications, optical imaging and nonlinear microscopy provide new opportunities for recording of metabolic and signalling information at the cellular level. Such understanding paves the way for new diagnostics, treatment, and drug development for a wide range of diseases.

Conventionally, optical imaging modalities are applied as standalone techniques each targeting one biomarker. However, recently it has been shown that diagnosis is significantly improved by combination of different contrast mechanisms in a multimodal approach. Hence, multimodal biomedical imaging, being considered the next generation technology within diagnostics, allows objective assessment of the status of disease, such as assisting staging and grading of cancerous lesions or tissue function monitoring. To transfer optical imaging technology into applications, systems would have to be compact, user-friendly, and fitted into endoscopes. Thus, photonic technologies and delivery systems for probing must be further developed.

Biophotonic Imaging Group - join the our research projects

Research vision

Our current research activities revolve around multimodal imaging enabling detection of relevant biomarkers for disease, by combining Optical Coherence Tomography (OCT), Two-Photon Excitation Fluorescence Microscopy (TPEFM), Three-Photon Excitation Fluorescence Microscopy, and lightsheet microscopy.

Research topics

OCT for morphology and optical properties

Optical coherence tomography (OCT) provides 2D or 3D scans of biological tissue in situ. OCT is already established as the gold standard in ophthalmic imaging. Other applications are emerging taking full advantage of high-speed frequency-domain OCT (FD-OCT), e.g., intravascular OCT, dermatologic OCT and OCT for endoscopy.

Our current research aims at combining OCT with TPEFM, see below. An important aspect of our current activities concerns light-tissue interactions and extraction of optical properties from OCT images. This can be seen as a novel, functional imaging modality that can dramatically improve diagnosis of disease. Recent results indicate that we can diagnose melanoma with 96% specificity and 97% sensitivity (compared to 72%/73% with current standard methods).

Two-photon excitation fluorescence microscopy for metabolic information

Two-photon excitation fluorescence microscopy (TPEFM) provides molecular and biochemical information arising from endogenous or exogenous fluorophores. Assessment of metabolites, such as NADPH, allows direct insight into the metabolic state of cells. Because the wavelength of the ultrashort pulse required for excitation is in the NIR region, where tissue scatters significantly less, the penetration depth is improved compared to light in the visible spectrum. Moreover, TPEFM can probe second-harmonic generation (SHG) that provides additional structural information.

Three-photon fluorescence microscopy using near-infrared light (1200 – 1500 nm) for excitation offers great promise to improve both the imaging depth and the signal to noise ratio in respect to single- and two-photon excited fluorescence microscopy and has been shown to be able to image in vivo brain tissue down to a depth of 1.2 mm. The added benefit of this excitation scheme is the simultaneous presence of second harmonic (SH), third harmonic (TH), two-photon fluorescence, and three-photon fluorescence signals from different fluorophores and structures.

Our current research aims at combining TPEFM with OCT/OCM on a microscope platform to investigate biomarkers relevant for bladder cancer. In vivo examination of bladder cancer requires developing novel light delivery probes, which would fit within existing endoscopes. Current efforts entail MEMS-based scanning probes and specialised delivery fibres for in-fibre dispersion compensation.

Our future research activities aim at deploying novel short pulsed fibre lasers for 2PM/3PM imaging applied to small animals studying brain function in vivo.

Single plane illumination microscopy for speed

(Image reproduced from "Integrated single- and two-photon light sheet microscopy using accelerating beams" by Piksarv et al., Sci. Rep. 2017)

Single plane illumination microscopy (SPIM), also known as lightsheet microscopy, overcomes the speed limitation in conventional TPEFM point-scanning techniques. In addition, since only the plane of interest is illuminated at each time point, risk of photo-toxicity is significantly reduced. In our current research, we aim at implementing TPEF Single Plane Illumination Microscopy (TPEF-SPIM) into endoscopes, both using miniaturised phase masks and all-fibre solutions.

Group Leader

Peter E. Andersen

Peter E. Andersen Groupleader, Senior Researcher Department of Health Technology Mobile: +45 22454557

Konstantinos Karageorgos

Master Thesis (2024)

Stefan M. Jensen

Master Thesis (2024)

Lars R. Lindvold

Faculty (2020-2023)

Maria Pedersen

Master Thesis (2023)

Dominika Melczer

Master Thesis (2023)

Sofie Degn

Bachelor Thesis (2023)

Josephine Schwarz

Bachelor Thesis (2023)

Lasse Bo Mortensen

Bachelor Thesis (2023)

Ali Mohebi

Visiting PhD Student (2022)

Dominik Marti

Faculty (2014-2022)

Monika Kupska

Master Thesis (2022)

Oriol Vidal Casassayas

Master Thesis (2022)

Keqing Dai (Sunny)

Master Thesis (2022)

Magdalena Skowyra

PhD Student (2019 – 22)

Michael Søndergaard Nørbo Madsen

Bachelor Thesis (2022)

Merle Loop

Diploma Thesis (2022)

Peter Groth Stounbjerg

Master Thesis (2022)

Madhu Veettikazhy

PhD Student (2018 – 21)

M. Tahir Jamal

Postdoc (2020 – 21)

Mahmoud Tawfieq

Postdoc (2020)

M. Tahir Jamal

PhD Student (2017 – 20)

Joana Kira Besecke

Master Thesis (2021)

Kristian Julius Moltved

Master Thesis (2021)

Rasmus Tue Nielsen

Master Thesis (2021)

Morgane Cecile Zimmer

Master Thesis (2021)

Vasiliki Koulianou

Master Thesis (2021)

Hafeez Ul Hassan

Postdoc (2019 – 20)

Björn-Ole Meyer

PhD Student (2017 – 20)

Anja Lykke Borre

Master Thesis (2020)

Adrianna Rokosa

Master Thesis (2020)

Lærke K. Larsen

Master Thesis (2020)

M. Pilar J. Stella

Master Thesis (2019)

Ida Videcrantz

Master Thesis (2019)

Sofie Tidemand-Lichtenberg

Bachelor Thesis (2019)

Rikke Aasbjerg

Master Thesis (2018)

Kira Schmidt

Master Thesis (2017)

Georgios Koukos

Master Thesis (2016)

Konstantinos Dimopoulos

Master Thesis (2016)

Mathias Christensen

Master Thesis (2015)

Faisal Kamran

PhD Student (2011 – 2015)


The group offices are located in building 345B at "Ørsteds Plads". In building 349, just across the square ("Plads"), we have our state-of-the-art photonics and bio-imaging lab.

Official visiting address is:

Biophotonic Imaging Group
Department of Health Technology
Ørsteds Plads, Building 345B
Technical University of Denmark (DTU)
DK-2800 Kgs. Lyngby