Teaching in courses:
Research Group
Nanofluidics and bioimaging
Group leader: Rodolphe Marie
The Nanofluidics and Bioimaging group is part of the OASIS section.
As an interdisciplinary research group, we focus on experimental nanofluidics for the manipulation and imaging of biologically relevant particles such as macro-molecules, lipid vesicles, metaphase chromosomes and cells.
Our goal is to provide new tools for the life sciences based on a combination of micro-/nanofluidics, biophysics, and several optical microscopy techniques.
Applications
- Enzymatic synergy
- Liquid biopsy
- Colloids
- Cancer study
- Cellular transport
Projects
Ongoing research projects.
Single molecule microscopy of enzymatic synergy
NNF BioSAP grant
Single molecule localization microscopy of enzymes
We study the behaviour of cellulases enzymes on cellulose nanofibers using TIRF microscopy.
Collaboration with:
Jonas Nyvold Pedersen at DTU Health Tech.
Peter Westh at DTU Bioengineering.
Kim Borch at Novozymes.
Next generation biocolloids analysis for diagnostics
NNF Nerd grant
Nanofluidic platform for the isolation and study of biocolloids
Nanofluidics and microfluidics for the purification of biocolloids from liquid biopsy. Fluorescence microscopy and label-free microscopy.
Single Molecule microscopy of colloids
Part of the EU ITN Supercol.
As part of the project EU ITN Supercol, we use single molecule localization microscopy, one of the super-resolution microscopy approaches, to study the functionalization of colloids with DNA and proteins. In particular we study how dielectric colloids may disturb the imaging using experimental and numerical approaches.
Microfluidic platform for cancer cells in gradients
Part of the NNF synergy Migragen.
A reversible microfluidic platform for gradients
We design and fabricate a microfluidic platform able to create multiple chemical gradients over cells. The platform has a reversible sealing to allow for further analysis of the cells. It started as the project Micrograd.
In collaboration with:
Stine Falsig Pedersen (Copenhagen University).
Albin Sandelin (Copenhagen University).
Utte Resch-Genger (BAM, Berlin).
Microscopy for peptide transport studies
part of the NNF challenge center Citbio.
As part of the NNF challenge center Citbio, we use live-cell microscopy of lipidated peptide transport in gut epithelial models.
Optical mapping of DNA in nanofluidics
Using nanofluidics to visualize fluorescence barcodes on genomic DNA. Combined with microfluidics to trap single cells and extract the genomic DNA.
See our past work in the EU project Cellomatic under 'Publications'.
Group Leader
Rodolphe Marie Groupleader, Associate Professor Department of Health Technology Phone: +45 45255676 rcwm@dtu.dk
Rodolphe Marie received his PhD from DTU in 2004 where he worked with microcantilever sensors in the group of Anja Boisen at the Department of micro and nanotechnology. He was a Post Doctoral fellow in Lund, Sweden, at the department for solid-state physics with Jonas Tegenfeldt and Fredrik Höök working with microfluidics and localized plasmon sensors. Back at DTU in 2008 he joined the group of Anders Kristensen where he worked with nanofluidics. He was appointed Assistant Professor at DTU Nanotech in 2008, Associate Professor in 2012 and group leader in 2017.
PhD
Aimilia Nousi Student Students s205640@student.dtu.dk
PhD
Masih Fahim Student Students s206396@student.dtu.dk
PhD
Teun A. P. M. Huijben Student Students s206286@student.dtu.dk
PhD
Shanil Durgeshkumar Gandhi Student Students s206556@student.dtu.dk
PhD
Pavlina Senikoglou PhD student Department of Health Technology pavse@dtu.dk
PhD
Corentin Cardot PhD student cordot@dtu.dk
Former PhD students
Adam C. Hundahl
Martin K. Rasmussen
Former MSc students
Philipp Willmer
Sofie Tidemand-Lichtenberg
Lu Chen
Sofie Tidemand-Lichtenberg
Lu Chen