Keith Moss

PhD Project by Keith Moss

Name: Keith Henry Moss            
Project Title: CodeSphere - Molecular encoding of nanoparticles for targeted cargo delivery
Group: T-cells & Cancer
Supervisor: Prof. Sine Reker Hadrup

Project Description:
The CodeSphere platform technology represents a unique strategy to generate and screen for optimized nanoparticle (NP) formulations in a high-throughput manner. The novelty in this proposed technology is the use of a DNA barcode as a unique NP identifier. Lipid nanoparticles (LNPs) will be tagged with a unique DNA barcode encoding for and identifying the particular composition.

Large, diverse, DNA-encoded NP libraries will be generated which can then be screened on cells in a single-tube assay. This allows the simultaneous assessment of thousands of different NP formulations for the most effective delivery of therapeutic cargo.

This technology would offer a unique strategy to identify optimal NP formulations for delivery of cargo in a variety of applications. Although this approach is focused primarily on the high-throughput optimization of therapeutic NPs in regard to cancer, it has the potential to be extended to other NP-based applications ranging from vaccine development to diagnostics and imaging. A primary application of this screening platform would be the development of a NP system, for the delivery of a tumor-specific chimeric antigen receptor (CAR) or T-cell receptor (TCR), capable of specifically targeting T-cells in vivo. This could potentially transform an expensive, patient-specific therapy to a generic and widely-available treatment strategy, without the need for patient T-cell gene modification and expansion ex vivo.


Sine Reker Hadrup
Sektionsleder, Professor
DTU Sundhedsteknologi
35 88 62 90