Nanofluidics and Bioimaging

Nanofluidics and Bioimaging is part of the NanoBio Light-Systems section.

As an interdisciplinary research group, we focus on experimental nanofluidics for the manipulation and imaging of biologically relevant particles such as macro-molecules, lipid vesicles, metaphase chromosomes and cells.

Our goal is to provide new tools for the life sciences based on a combination of micro-/nanofluidics, biophysics, and various imaging techniques.

Nanofluidics traps and arrays: we establish nanofluidics devices to array single molecules and soft matter nanoparticles. (Left) In our activity in optical mapping of genomic DNA, we made a device arraying single DNA molecules with no attachement to surfaces. (Right) A new method to trap and characterize soft-matter nanoparticles in a salt gradient using competing diffusiophoresis and -osmosis.

     

Microfluidic reactors: (Left) A microfluidic reactor for performing enzymatic reactions on single metaphase chromosomes. This activity supported by the EU project Chromavision and the NNF Synergy project ChromoCapture. (Right) We use 3D printing and PDMS prototyping for microfluidics. In the project MicroGrad supported by NNF, we aim to create microfluidic gradients for single cell studies.

     

Super-resolution imaging: We develop super-resolution fluorescence microscopy for tracking single particles in live cells (NNF challenge center CitBio). We also use TIRF imaging and SD imaging for single molecule studies (NNF biotech grant on Cellulases) and soon our home-built structured illumination microscope (EU ITN SuperCol project).

nanofluidics and bioimaging

 

 

Recent articles

 

Camera-based localization microscopy optimized with calibrated structured illumination', Communications Physics (2021) Full text and the related Nature Communities blog

 

Label-Free Sensing of Biorecognition on Liposomes', ACS Sensors, (2020) Full text

 

'Size and surface charge characterization of nanoparticles with a salt gradient’, Nature Communications, (2020) Full text

 

‘Single-molecule DNA-mapping and whole-genome sequencing of individual cells’, PNAS (2018) Full text

 

‘Concentrating and labeling genomic DNA in a nanofluidic array’, Nanoscale, (2018) 


Contact

Rodolphe Marie
Groupleader, Associate Professor
DTU Health Tech
+45 45 25 56 76