Fluidic Array Systems and Technology

The Lab works with digital assays and barrier/organ modelling

 

The goal of barrier/organ modelling is to create tissue and organ models using combination of microfluidicsstructured hydrogelsor stackable hydrogels systems. Organ/tissue models are the intestine, liver, blood vessels. The goal is to combine these cell models into an easy to use, flexible and physiologically relevant system. The lab investigates several high throughput appraoches that models oral drug delivery including the intestine, blood and liver. Our long-term goal is to have an easy to use mix and match approach to assemble different organs and barriers. Interests is presently centred to build linked tissue models for testing drug delivery (DNRF-IDUN project) and viral infectious routs (MSCA-ITN Organovir project). A long-term interest it to investigate how different tissues interact with each other. 

 

The second activity regards single molecule counting and digital analysis. The motivation is that an ever increasing need to analyse large sample volumes for very few molecules. It is relevant for serum analysis pf Alzheimer’s disease and cancer screening, monitoring and diagnosis. The technical approach is to create droplets on surface (so called droplet arrays). These droplets are used to capture single molecules and to detect the molecules using enzymatic reactions. Currently we are working on different ways to improve assays sensitivity. Multiplexing is central and is investigate using 3D printed fluidics.  The latter is usable for Alzheimer’s disease diagnostic (MSCA-ITN BBDiag). 

 

A new usage of droplets arrays is currently being explored. High throughput screening is today cumbersome, expensive and technically challenging. The aim for a National research council is to increase the speed of screening (even quicker than emulsion droplets) and to use much simpler instrumentation. 

 

List of publications can be found here

Contact

Martin Dufva
Groupleader, Associate Professor
DTU Health Tech
+45 51 33 37 53