JP Narag

PhD project by Jadze Princeton Narag

Name: Jadze Princeton Narag
Project Title: Visualizing transport at biological barriers with high temporal and spatial detail
Group: Imaging of Subcellular Life
Supervisor(s): Emil Boye Kromann, Niels Bent Larsen, Jens E. Wilhjelm

Project Description:
Our goal is to enable visualization of peptides with distinct properties as they traverse a model of the intestinal barrier, thus providing a platform for obtaining a deeper understanding of the selective transport processes involved in the uptake of drugs/nutrients. We will construct a state-of-the-art lattice light-sheet fluorescence microscope with adaptive optics capability for live-sample imaging, and to optimize it for use with organ-on-a-chip models of the intestine. This imaging-based method of inquiry is also generalizable to investigations of many other biological processes and other organ-models.

Perspective:
Uptake from the intestine to the blood is mediated by an intestinal barrier which exhibits some selectivity, i.e. mechanisms in the intestinal barrier define which compounds are absorbed and which are not. We want to uncover what and how different types of peptides pass from the intestines to the bloodstream and to answer questions like: Do they pass through the cells? Do they pass between the cells? If they do not pass, where do they stop? Do they pass in vesicles? If so, which vesicle type? Learning more about these mechanisms and understanding what drives them will help in the design and development of efficient delivery of oral drugs to different target cells.

 

 

Kontakt

Jadze Princeton Casilana Narag
Ph.d.-studerende
DTU Sundhedsteknologi

Kontakt

Emil Boye Kromann
Gruppeleder, Lektor
DTU Sundhedsteknologi
23 37 22 38

Kontakt

Niels Bent Larsen
Sektionsleder, Professor
DTU Sundhedsteknologi
45 25 81 61

Kontakt

Jens E. Wilhjelm
Professor, gruppeleder
DTU Sundhedsteknologi
45 25 38 86