Asli Aybike Dogan

PhD project by Asli Aybike Dogan

Name: Asli Aybike Dogan
Project Title: Developing a three-dimensional (3D) co-culture system with scalable and stackable defined extracellular matrix (ECM)-mimetic hydrogels for high-throughput screening (HTS)
Group: Fluidic Array Systems Technology (FAST)
Supervisor: Assoc. Prof. Martin Dufva

Project Description:
This PhD project is part of MSCA-ITN OrganoVIR project, in the field of organoid technology to make organoids a superior model for virus research to prevent future infections, to test vaccines and, to replace animal use in virology. The research project aims to develop a 3D co-culture system as assembling different organs and barriers (e.g., gastrointestinal tract, blood-brain-barrier), with scalable and stackable controlled/patterned ECM-mimetic hydrogels for HTS of viruses as an alternative in vitro platform for virus studies. The main objectives of this project:

To build 3D-printed co-culture systems with flexible hydrogel matrices for HTS of viruses and vaccines/drugs.
To place organoids behind a basement membrane to simulate coupled organ systems.
To test the platform for the propagation of Enteroviruses (EV).

Many viruses cause infectious diseases can seriously be threatening human health due to their highly contagious nature. Conventional preclinical models for viral infections are typically two-dimensional (2D) or rely on animal models to trace and treatment. While 2D models can provide important information on early biological responses and are suitable for HTS drug screening, they have poor predictive capabilities. Moreover, while existing animal models can capture the complex physiology and interactions of in vivo tissues, they often fail to recapitulate human responses due to species-specific differences. Therefore, it has become a necessity to overcome these limitations and develop 3D humanized models that are able to capture complex physiological responses in vitro.



Martin Dufva
Groupleader, Associate Professor
DTU Health Tech
+45 51 33 37 53